Dietary Gum Arabic Alleviates Carbon Tetrachloride Induced Liver Fibrosis in Wistar Rats
Mohammed Hamid *
Faculty of Veterinary Sciences, University of Nyala, Sudan.
Yassin Abdulrahim
Faculty of Veterinary Sciences, University of Nyala, Sudan and College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China.
Abdelnasir A
Faculty of Veterinary Sciences, University of Nyala, Sudan and Department of Pathology and Sample Management, Veterinary Laboratories Division, Animal Wealth Sector, AbuDhabi Agriculture and Food Safety Authority (ADAFSA), Abu Dhabi, UAE.
Khalid M. Mohammedsalih
Faculty of Veterinary Sciences, University of Nyala, Sudan.
Nagmeldin A. Omer
College of Allied Medical Sciences, University of Nyala, Sudan.
Juma Ahamed Abaker
Faculty of Veterinary Sciences, University of Nyala, Sudan.
Hassan M. A. Hejair
Faculty of Veterinary Sciences, University of Nyala, Sudan.
Tamour Elkhier
Faculty of Veterinary Sciences, University of Nyala, Sudan.
Tagwa Norain Mahmoud
Faculty of Veterinary Sciences, University of Nyala, Sudan.
Hidaia B. Zolain
Faculty of Veterinary Sciences, University of Nyala, Sudan.
*Author to whom correspondence should be addressed.
Abstract
Aims: This study was carried out to investigate the effect of Gum Arabic (GA) on liver fibrosis induced by carbon tetrachloride (CCl4) in Wistar rats.
Methods: Rats were randomly divided into three groups (each group: n=8). Group 1 served as untreated control with just 2 ml/kg of olive oil. Group 2 administered only by intraperitoneal (I.P) injection of CCl4 dissolved in olive oil at a dose of 2 ml/kg body weight twice a week for 7 week. Group 3 administered in additional to CCl4 a basal diets containing GA (5%).
Results: The outcomes of the current study exhibited that, CCl4 elevated the serum levels of liver enzymes, malondialdehyde (MDA) and hydroxyproline. In addition, CCl4 increase the expression of inflammatory cytokines TLR-4, TNF-α, IL-6, IL-β1and MCP-1or fibrotic markers cytokines; TGFβ1and α-SMA while decreasing the activity of T-AOC, SOD and GSH-Px . Administration of GA effectively attenuated these changes, in additional to promotion of antioxidants enzymes and reducing the collagen deposition and hepatocytes necrosis.
Conclusion: These results suggested that GA has antifibrotic properties and could be used as potential dietary agent against CCl4-induced liver fibrosis in rats.
Keywords: Gum Arabic, carbon tetrachloride, liver, fibrosis, rats