Serological Investigation of Canine Ehrlichiosis in Khartoum State, Sudan

Mona F. Alshiekh Awooda

Department of Pathology, College of Veterinary Medicine, University of Bahri, Sudan.

Maaly Z. M. Abdalla

Department of Internal Medicine, Pharmacology and Toxicology, College of Veterinary Medicine, University of Bahri, Sudan.

El Ayis A. Abubaker *

Department of Internal Medicine, Pharmacology and Toxicology, College of Veterinary Medicine, University of Bahri, Sudan.

Wael I. Mursal

Department of Pathology, College of Veterinary Medicine, University of Bahri, Sudan.

*Author to whom correspondence should be addressed.


Abstract

This study was extended for 2 years and covered different seasons of the years 2021 and 2022. The study was aiming at investigating the prevalence of Canine Ehrlichiosis disease among dogs. A total of 599 dogs from different breeds, ages and different localities of Khartoum State were investigated for the presence of Canine Ehrlichiosis. According to the results of E. canis Ab – Bio Note- Canine Rapid Test for the detection of Canine Ehrlichiosis, the prevalence of Canine Ehrlichiosis in Khartoum State was 5.6%. Male and female dogs were susceptible for Canine Ehrlichiosis with the prevalence of 2.8% and 2.8% respectively. Dogs of all ages were susceptible for the disease. According to ANOVA analysis, the mean infection in autumn is higher than that in winter or summer but there was no statistical significant difference (p-value= 0.685) in Canine Ehrlichiosis among the three seasons. According to Chi-2 test, cross breed dogs were more susceptible for Canine Ehrlichiosis in Khartoum State. Eighty-eight-point two percent of Canine Ehrlichiosis cases respond for the treatment trials with Doxycycline. This is the first study concerning investigation of Canine Ehrlichiosis in Khartoum State. Vaccination and Deworming programs against different diseases are recommended for dogs in Khartoum State.

Keywords: Canine, Khartoum State, ehrlichiosis, protozoal infestation, Sudan


How to Cite

Awooda, Mona F. Alshiekh, Maaly Z. M. Abdalla, El Ayis A. Abubaker, and Wael I. Mursal. 2023. “Serological Investigation of Canine Ehrlichiosis in Khartoum State, Sudan”. Asian Journal of Research in Animal and Veterinary Sciences 6 (2):158-66. https://doi.org/10.9734/ajravs/2023/v6i2242.

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References

Dagnone AS, Morais HAS, Vidotto MC, Jojima FS, Vidotto O. Ehrlichiosis in anemic, thrombocytopenic, or tick-infested dogs from a hospital population in South Brazil. Vet. Parasitol. 2003;117(4): 285-290.

Nakaghi ACH, Machado RZ, Tinucci-Costa M, Andre MR, Baldani CD. Canine ehrlichiosis: clinical, hematological, serological and molecular aspects. Cienc Rural., 2008;38(3): 766-770.

Faria JLM, Dagnone AS, Munhoz TD, Joao CF, Pereira WAB, Machado RZ et al. Ehrlichia canis morulae and DNA detection in whole blood and spleen aspiration samples. Rev. Bras. Parasitol. Vet. 2010; 19(2): 98-102.

Vieira RFC, Biondo AW, Guimaraes AMS, Santos AP, Santos RP, Dutra LH et al. Ehrlichiosis in Brazil. Rev. Bras. Parasitol. Vet. 2011;20(1): 1-12.

Anziani OS, Ewing SA, Barker RW. Experimental transmission of a granulocytic form of the tribe Ehrlichieae by Dermacentor variabilis and Amblyomma americanum to dogs. Am. J. Vet. Res. 1990;51:929-931.

Little SE, O’Connor TP, Hempstead J, Saucier J, Reichard MV, Meinkoth K, Meinkoth JH, Andrews B, Ullom S, Ewing SA, Chandrashekar R. Ehrlichia ewingii infection and exposure rates in dogs from the southcentral United State. Vet Prasitol. 2010;172: 355-360.

Troy G C, Forrester S. Canine ehrlichiosis. In: Green CE (Ed), Infectious diseases of the dog and cat. Philadelphia: W.B. Sanders. Co., 1990;404-418.

Castro MB, Machado RZ, Tomaz de Aquino LPC, Alessi AC, Costa MT. Experimental acute canine monocytic ehrlichiosis: clinicopathological and immunopathological findungs. Vet. Parasitol. 2004;5:119(1):73-86.

Trautwein C, Boker K, Manns MP. Hepatocyte and immune system: acute phase reaction as a contribution to early defence mechanisms. Gut. 1994;35(9): 1163-1166. P. Mid: 7525420.

Gabay C, Kushner I. Acute-phase proteins and other systemic responses to inflammation. N Engl J Med. 1999;340(6): 448-454.

Ceron LL, Eckersall PD, Martinez-Subiela S. Acute phase proteins in dogs and cats: current Knowledge and future perspectives. Vet. Clin. Pathol. 2005;34(2): 85-99.

Dahmani M, Davoust B, Tahir D, Fenollar F, Mediannikov O. Molecular investigation and phylogeny of Anaplasmataceae species infecting domestic animals and ticks in Corsica, France. Parasites and Vectors. 2017;(10):302.

Ewing S A. Canine ehrlichiosis. Adv. Vet. Sci. Comp. Med., 1969;(13):331-353.

Neer TM Harrus S. Canine monocytotropic ehrlichiosis and neorickettiosis (E. canis, E. chaffeensis, E. ruminantium, N. sennetsu, and N. risticii infections). In: Greene CE (Ed), Infectious diseases of the dog and cat. 3rd ed. St. Louis, Missouri: Saunders Elsevier. 2006; 203-216.

Waner T, Harrus S, Bark H, Bogin E, Avidar Y, Keysary A. Subclinical canine ehrlichiosis (ehrlichia canis) in experimentally infected beagle dogs. Vet. Parasitol. 1997;69 (3-4): 307-317.

Troy GC, Forrester SD. Canine ehrlichiosis. In: Green CE (Ed), Infectious diseases of the dog and cat. Philadelphia: W.B. Sanders. Co., 1990;404-418.

Nyindo M, Huxsoll D L, Ristic M, Kakoma I, Brown JL, Carson CA, Stephenson EH. Cell-mediated and humoral immune responses of German Shepherd Dogs and Beagles to experimental infection with Ehrlichia canis. American Journal of Veterinary Research., 1980;(41): 250-254.

Macieira D, MessIck J, Cerguera A, Freire I, Linhares G, Almeida N. et al. Prevalence of Ehrlihia canis infection in thrombocytopenic dogs from Rio de Janeiro, Brazil. Vet. Clin. Pathol. 2005;(34): 44-48.

Khaled Mohamed El-Dakhly, Magdy M Tawfik Amany, Samir Aboshinaf, Lilian N Mahrous, Waleed M Arafa. Detection of Anaplasmosis and Ehrlichiosis in Blood of Owned Dogs in Alexandria, Northern Egypt. Adv. Anim. Vet. Sci., 2021;9(9): 1383-1389.

Tanikawa A, Labruna MB, Costa A, Aguiar DM, Justiniano SV, Mendes RS, Melo, ALT, Alves CJ, Azevedo SS. Ehrlichia canis in dogs in a semiarid region of Northeastern Brazil: Serology, molecular detection and associated factors. Res. Vet. Sci., 2013;(94): 474-477.

Dahmani M, Davoust B, Sambou M, Bassene H, Scandola P, Ameur T, Raoult D, Fenollar F, Mediannikov O. Molecular investigation and phylogeny of species of the Anaplasmataceae infecting animals and ticks in Senegal. Parasit. Vectors. 2019;(12): 495.

Adamu M, Troskie M, Oshadu DO, Malatji DP, Penzhorn BL, Matjila PT. Occurrence of tick-transmitted pathogens in dogs in Jos, Plateau State, Nigeria. Parasit. Vectors. 2014;(7): 119. 3305-3307.

Dhliwayo S, Chihambakwe B, Taonezvi K, Chikerema SM, Tivapasi MT, Pfukenyi DM. Seroprevalence of canine ehrlichiosis and microscopic screening for canine babesiosis in dogs in Harare, Zimbabwe, Vet. Med. Int., 2019;(10).11554130210- 7.

Almazan C, Gonzalez-Alvarez VH, de Mera IGF, Cabezas-Cruz A, Rodriguez-Martinez R, de la Fuentec J. Molecular identification and characterization of Anaplasma platys and Ehrlichia canis in dogs in Mexico. Ticks Tick-borne Dis. 2016;(7): 276-283.

Selim Abdelfattah, Said Ahmed Shimaa, Galila Elsayed. Epidemiological and molecular diagnosis of Ehrlichia canis infection among dogs. Benha. Vet. Med. J., 2019;(37): 169-171.

Angelou A, Gelasakis AI, Verde N, Pantchev N, Schaper R, Chandrashekar R, Papadopoulos E. Prevalence and Risk Factors for Selected Canine Vector-Borne Diseases in Greece. Parasites. Vectors. 2019;(12), 283.

Muhammad Umair Aziz, Sabir Hussain, Baolin Song, Hammad Nayyar Ghauri, Jehan Zeb, Olivier Andre Sparagano. Ehrlichiosis in Dogs: A Comprehensive Review about the Pathogen and Its Vectors with Emphasis on South and East Asian Countries. Vet. Sci. 2023;10 (1): 21.

Elisa Brandão Guedes et al. Canine ehrlichiosis: prevalence and epidemiology in northeast Brazil Paula. Rev. Bras. Parasitol. Vet. 2015;24(2):115-21.

Bowman D, Little SE, Lorentzen L, Shields J, Sullivan MP, Carlin EP. Prevalence and Geographic Distribution of Dirofilaria Immitis, Borrelia Burgdorferi, Ehrlichia Canis, and Anaplasma Phagocytophilum in Dogs in the United States: Results of a National Clinic-Based Serologic Survey. Vet. Parasitol. 2009;160, 138–148.

Gray J, Dantas-Torres F, Estrada-Pena A, Levin M. Systematics and ecology of the brown dog tick, Rhipicephalus sanguineus. Ticks Tick Borne Dis. 2013;4(3):171–80.

Ángel Sainz, Xavier Roura, Guadalupe Miró, Agustín Estrada-Peña, Barbara Kohn, Shimon Harrus, Laia Solano-Gallego. Guideline for veterinary practitioners on canine ehrlichiosis and anaplasmosis in Europe. Parasit. Vectors. 2015;(8): 75.

Kevin Horecka, Steve Porter E, Susan Amirian, Ellen Jefferson. A decade of Treatment of Canine Parvovirus in an Animal Shelter: A Retrospective Study. Animals (Basel).2020;10(6): 939.

Kathryn J Sarpong, Jennifer M Lukowski, Cassandra G Knapp. Evaluation of mortality rate and predictors of outcome in dogs receiving outpatient treatment for parvoviral enteritis. J. Am. Vet. Med. Assoc., 2017;251(9):1035-1041.